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. Author manuscript; available in PMC: 2016 May 17.
Published in final edited form as: Immunity. 2015 Nov 10;43(5):923–932. doi: 10.1016/j.immuni.2015.10.009

Figure 3. Pannexin-1 is critical for ATP-induced pyroptosis induced by cytosolic LPS.

Figure 3

(A–C) BMMs were primed overnight with LPS and then transfected with ultrapure S. minnesota RE595 LPS packaged with DOTAP.

(A) Wild-type BMMs were treated with DMSO, CBX (50 µM), bafilomycin A (50 nM), BFA (1 µM), 18GA (50 µM), FFA (50 µM), glibenclamide (100 µM), Gd3 (100 µM), probenecid (100 µM) and trovafloxacin (100 µM), and extracellular ATP was measured 15 min after LPS transfection.

(B) Cytotoxicity was measured 2 hrs after LPS transfection. Treatment with inhibitors was as in (A).

(C) ATP release was measured 15 min after LPS transfection, and cytotoxicity was determined 2 hrs after transfection in wild-type and Panx1−/− BMMs.

(A–C) Data are representative of at least 3 experiments. Graphs show mean ± SD of triplicate cultures, * p<0.05, ** p<0.01. See also Figure S4 and S5.