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. Author manuscript; available in PMC: 2017 Jan 13.
Published in final edited form as: J Am Chem Soc. 2015 Dec 28;138(1):289–299. doi: 10.1021/jacs.5b10494

Figure 6.

Figure 6

Visualization of persulfides and polysulfides in normal fibroblasts in response to exogenous cystine treatment. (A) Photomicrographs of human lung fibroblasts cultured ±200 µM cystine for 1 h and labeled by the CN biotin tag-switch method. Nuclei were stained with DAPI. (B) Western blot analysis of proteins from (A) shows a similar labeling intensity in cells grown ± cystine supplementation. GAPDH was used as a loading control (bottom). (C) Representative photomicrographs show a clear increase in fluorescence in cells treated with cystine when probed with the SSP4 reagent. Nuclei were stained with Hoechst. Scale bar: 20 µm.