Table 1.
Qualitative AcpH substrate selectivity for carrier proteins. AcpH homolog activity was determined through overnight reaction with crypto-CP except in the case of holo-SoAcpP. A significant reduction in fluorescence of AcpH-treated CP from controls (Supplementary Figures 2–9, 11–14) or Urea-PAGE gel-shift (Supplementary Figure 10) are labeled as active “+”.
| Carrier Protein | Enzyme | |||||
|---|---|---|---|---|---|---|
| Organism | Name | PaAcpH | PfAcpH | CyAcpH | SoAcpH | |
|
|
||||||
| FAS | E. coli | AcpP | + | + | + | − |
| P. aeruginosa | AcpP | + | + | + | − | |
| S. oneidensis | AcpP | + | + | + | − | |
| P. falciparum | ACP‡ | + | + | + | − | |
| M. tuberculosis | AcpM | + | + | + | − | |
| M. tuberculosis | MAS | − | − | − | − | |
|
|
||||||
| PKS | S. coelicolor | ActACP | − | + | − | − |
| A. parasiticus | PksA | + | + | + | − | |
| G. fujikuroi | Pks4 | − | + | − | − | |
| L. majuscula | JamC | + | + | + | − | |
| L. majuscula | JamF | − | − | − | − | |
| P. agglomerans | AdmA | − | − | − | − | |
|
|
||||||
| NRPS | P. agglomerans | AdmI | − | + | − | − |
| V. cholerae | VibB | − | − | − | − | |
| A. orientalis | CepK | − | − | − | − | |
| P. protogens | PltL | + | − | − | − | |
| P. syringae | SyrB1 | − | − | − | − | |
‡
P. falciparum ACP is derived from the apicoplast FAS-ACP.