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MCF2L689Gly does not activate Rac1. The MCF2L-689Gly variant does not activate Rac1. Hela cells were transiently transfected with wild-type MCF2L689Asp or the mutant MCF2L689Gly constructs. Rac1 protein was measured in cell lysates. Rac1-GTP was measured as described in methods section. Wild-type MCF2L689Asp but not MCF2L689Gly activates endogenous Rac1. Beta-actin was used as a loading control.
