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. Author manuscript; available in PMC: 2016 Mar 17.
Published in final edited form as: Traffic. 2015 Apr 28;16(7):773–786. doi: 10.1111/tra.12276

Figure 2. Addition of 3'UTR to integration plasmid rescues synthetic lethality with bik1Δ.

Figure 2

(A) Schematic depicting strategy for construction of TUB1+3'UTR-integration plasmids. Arrows indicate forward and reverse primers used for PCR, which include BamHI and SalI restriction sites for cloning. Restriction digest by BsaBI targets the plasmid for homologous integration into the TUB1 locus, as depicted. Dashed box delineates chromosomally-integrated plasmid. (B) Representative tetrad progeny of indicated crosses. Red boxes indicate bik1Δ TUB1::HIS3p:mRuby2-TUB1 progeny and bik1Δ TUB1+3'UTR::HIS3p:mRuby2-TUB1 progeny as assessed by marker analysis.