Fig. 2.

An isobologram for the combination of Ferulic Acid with piperine which generates a synergistic effect. a Isobologram illustration for maximal anti-immobility response which achieved in the forced swim and b in the tail suspension tests. The dose at which 50 % of treated mice achieved a maximal anti-immobility response (ID₅₀) for each drug is indicated on their respective axes. The two points below “the line of additivity” connecting the ID₅₀s of the individual drug represent the ID₅₀s of the combination, which are 10 mg/kg piperine and FA, and 3 mg/kg FA and piperine, respectively. The ID₅₀s of the combination as shown do not cross the line of additivity (a position above the line indicates antagonism)