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. 2016 Mar 17;11(3):e0151525. doi: 10.1371/journal.pone.0151525

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© 2016 Fajardo et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — A-F. Cellular localization, dose-response, and time-course of TQ-induced AR protein down-regulation in AR-expressing prostate cancer cells. AR protein levels determined by immunoblot in LNCaP (A) and LAPC4 (B) cells treated with 25 μM TQ or 25 μM AT for 4 d. (C) TQ dose-dependent reduction in AR protein levels in LNCaP cells treated with 4, 12.5, or 25 μM TQ for 4 d. (D) Immunoblot of time-dependent changes in AR protein levels from LAPC4 cells treated with 25 μM TQ for 24, 48, 72, and 96 h. Quantified AR protein levels are presented below each immunoblot (* P<0.05). (E-F) LNCaP cells were treated with vehicle control (E) or 25 μM TQ (F) for 4 d and AR protein expression was detected by immunocytochemistry.