Table 1. Primers and PCR conditions used to amplify the rrs gene of symbionts.

Organism	Primer name	Primer sequence (5’– 3’)	Amplicon size (bp) /Tm (°C)	References
Eubacteria	pA	5’—AGAGTTTGATCCTGGCTCAG—3’	1500 / 55	[84]
	pH	5’—AAGGAGGTGATCCAGCCGCA—3’
Acinetobacter	Acin1	5’- ACTTTAAGCGAGGAGGAGGCT—3’	426 / 58	[85]
	Ac	5’—GCGCCACTAAAGCCTCAAAGGCC—3’
Spiroplasma	16STF1	5’—GGTCTTCGGATTGTAAAGGTCTG—3’	964 / 55	[53]
	16STR1	5’—GGTGTGTACAAGACCCGAGAA- 3’
Wolbachia	199F	5’- TTGTAGCCTGCTATGGTATAACT—3’	864 / 52	[52]
	1994R	5’—GAATAGGTATGATTTTCATGT—3’
Midichloria mitochondrii	Midi-F	5’—GTACATGGGAATCTACCTTGC—3’	1100 / 56	[54]
	Midi-R	5’—CAGGTCGCCCTATTGCTTCTTT—3’