Fig 1. H₂O₂, high glucose, and Ang II inhibit phosphorylation of AMPK Thr-172 and PARP1 Ser-177 in HUVECs.

Western blot analysis of AMPK Thr-172 and PARP1 Ser-177 in HUVECs treated with concentrations of H₂O₂ (A), glucose (B), and Ang II (C) for 4 hr. (D-F) treated with H₂O₂ (100 μM), glucose (30 mM), or Ang II (100 nM) for the indicated times. Data are mean±SD ratio of phospho-PARP1 Ser-177 to total PARP1 and phospho-AMPK Thr-172 to total AMPK from 3 independent experiments. *p<0.05 compared to controls.