Fig 2. TES promoter demethylation and decreased NALM6 cell viability after decitabine exposure.

NALM6 cells were exposed to decitabine (0, 0.5 and 5.0 μM) for 24, 48, 72 and 96 hours. (A) TES qualitative RT-PCR: total RNA was mock (-) or reverse transcribed (+) before TES-specific amplification with exon-specific primers (exons 5 and 6). Expected PCR product sizes for cDNA and genomic DNA were 213 bp and 555 bp, respectively. TES expression levels, as measured by quantitative RT-PCR assay and calculated relative to PBL cDNA, are recorded under each lane. (B) Viable NALM6 cell number was measured by WST-1 assay (error bars are standard deviations).