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. 2016 Mar 17;11(3):e0151341. doi: 10.1371/journal.pone.0151341

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© 2016 Weeks et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 8 — Forty μg of protein lysate from transfected and amsacrine-treated NALM6 cells were immunoblotted. EGFP-TESTIN fusion protein was detected using rabbit anti-TESTIN polyclonal antibody (Abcam: ab78499), and results showed rapid expression after transfection. Anti-TP53 monoclonal antibody (Cell Signalling) and anti-CDKN1A antibody (Santa Cruz Biotechnology) demonstrate lack of TP53 or CDKN1A induction after transfection, but robust induction after amsacrine exposure. Anti-α actin antibody was used to confirm equal protein loading.