Fig 12. Affymetrix mouse Exon ST 1.0 analysis of gene expression changes as a result of loss of Meis1 in an HSC-enriched population.

Individual, litter-matched MxCre/Meis1^fl/fl and MxCre/Meis1^fl/+ mice were induced for Meis1 deletion with PolyI:C every 48 hours for 18 days. 7 days following the final injection, mice were euthanized, BM extracted and sorted for LSK cells by FACS. RNA was isolated from this HSC-enriched population and amplified prior to hybridization on an Affymetrix mouse Exon ST 1.0 gene expression array. Signal was normalized using Expression Console software and log2 summarization. Differentially expressed genes were identified using two-tailed t-test with multiple hypothesis correction. Changed pathways were interrogated by leading edge analysis. a) Validation of selected candidate target genes by Q-RT-PCR, using both sample RNA and two additional independent replicates. b) Leading edge gene-set enrichment analysis reveals enrichment of genes involved in cell cycle in MxCre/Meis1^-/- cells.