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. 2016 Mar 17;11(3):e0151279. doi: 10.1371/journal.pone.0151279

Fig 5. Delta in PNs is required for PN physiological plasticity.

Fig 5

(A) Schematic of the experimental protocol. (B) The relevant genotypes are shown on the cartoon of the olfactory circuit. In C through F female flies were exposed to 1% GA in paraffin oil or paraffin oil alone and removed from odor for one day prior to imaging. The traces in C, D and E depict the median ΔF/F for each genotype and condition over time. (The numbers on the x-axis are frames of 472 msec each). The black bar indicates the time of a 1.5 second GA pulse. Solid traces, GA exposed flies; dotted trace, air exposed flies; magenta traces, control; pale blue traces, Dl RNAi; purple traces, N RNAi. (F) ∫ΔF/F, plotted in log scale, of calcium influx of flies shown in C, D and E. Statistically significant comparisons are indicated by asterisks. All other biologically relevant comparisons were not statistically significant. The number of quantified glomeruli are: lane 3, 18; lanes 4, 25; control and Dα7-DN flies are the same as in Fig 2E. Control, Dl RNAi and Dα7-DN experiments were carried out at the same time. In D the Dl RNAi (pale blue) and Dα7-DN (blue, data from Fig 2) are superimposed and in E traces of Dl RNAi in PNs (pale blue) are superimposed with those of N RNAi in ORNs (purple, data from Kidd et al, 2015 [10]). Flies were MZ612-GAL4 UAS.GCaMP6s/Or82a-LexAGAD; UAS.GCaMP6s LexOP.dsRED with UAS.Dl shRNA (Dl RNAi), with UAS.Dα7-DN (Dα7-DN) or with neither (control) and MZ612-GAL4 UAS.GCaMP6s/Or82a-LexAGAD; UAS.GCaMP6s LexOP.dsRED with LexOP.N shRNA (N RNAi, data from Kidd et al., 2015 [10]).