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. 2016 Mar 17;11(3):e0151658. doi: 10.1371/journal.pone.0151658

Fig 7. Subcellular localization of AtSYP22 is altered in nhx5 nhx6.

Fig 7

(A) Subcellular localization of GFP:SYP22. The SYP22:GFP plasmid was co-transformed with the PVC marker mRFP:AtVSR5, TGN marker RFP:SYP61 or Golgi marker Man1:RFP into the leaf protoplasts of Arabidopsis, respectively. Fluorescence was visualized by a confocal laser scanning microscope. Bar = 10μm. (B) Quantification of the subcellular localization pattern of AtSYP22. The overlapping percentage of GFP:SYP22 signal with mRFP:AtVSR5, RFP:SYP61 or Man1:RFP, respectively, was determined from more than 100 protoplasts obtained from three independent experiments. Error bars represent SD, n = 3. ***P≤0.001, *P≤0.05, by t test.