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. 2016 Mar 17;11(3):e0151621. doi: 10.1371/journal.pone.0151621

Fig 2. Sdc can promote NMJ growth when expressed in either neurons or muscles.

Fig 2

(A) Schematic diagram of the wildtype Sdc construct (Sdc-wt) used in previous studies and the myc-tagged full-length Sdc construct (Sdc-FL) used in this study. HS—heparan sulfate sidechains; Ecto—extracellular domain; Tm—transmembrane domain; C1—Conserved Domain 1; V—Variable Domain; C2—Conserved Domain 2. *—site of 5xMyc tag used to detect construct expression. (B) Presynaptic or postsynaptic overexpression of either Sdc-wt or Sdc-FL causes a significant increase in the number of boutons at the NMJ on muscles 6/7, ranging from an 11% increase (presynaptic expression of Sdc-wt) to 19% (postsynaptic expression of Sdc-wt; **—p < .01) (C) Expression of either Sdc-wt or Sdc-FL in a Sdc mutant background significantly rescues the size of the 6/7 NMJ (**—p < .01, *—p < .05). These constructs are indistinguishable when expressed presynaptically, but Sdc-FL (cloned into the attp2 site) generates a more complete rescue than Sdc-wt when expressed postsynaptically. Sdc-wt expression data were published previously [5].