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. 2016 Mar 17;11(3):e0151621. doi: 10.1371/journal.pone.0151621

Fig 3. The cytoplasmic and extracellular domains are required for Sdc function.

Fig 3

(A) Schematic diagram of the Sdc constructs used in this study. The Tm domain of the TM swap construct was made from human platelet derived growth factor receptor (hPDGFR). *—site of 5xMyc tag used for construct detection. (B) Muscle overexpression of the UAS-Sdc constructs shown in (A) using the 24B-Gal4 driver. Overexpression of either Sdc-FL or TM Swap generated a significant increase in the number of boutons per NMJ when compared to Canton S controls (p < .01 n = 21; p < .05 n = 21). Overexpression of ΔCyto caused a significant reduction in the number of boutons per NMJ (p < .05; n = 23). (C) Sdc mutants had a significant decrease in boutons per NMJ as described previously [5]. The UAS-Sdc constructs shown in (A) were expressed in muscles in a Sdc mutant background (P2377/Df48; ubi-Sara) using the 24B-Gal4 driver. Both Sdc-FL and TM Swap constructs generated a significant rescue of the number of boutons per NMJ when compared to Sdc mutants (p < .01 n = 40; p < .01 n = 23).