Table 1. Informative subsets of parental, chimeric, and designed GH5 cellulases.
| Parents (P) | ABCD EFG H | SCHEMA E | m | TA50 (°C)1 | T50 (°C)2 | Are3* |
|---|---|---|---|---|---|---|
| P1 1 1 1 1 1 1 1 1 | 0 | 0 | 80 ± 0.9 | 79 ± 0.4 | 1.17 ± 0.06 | |
| P2 2 2 2 2 2 2 2 2 | 0 | 0 | 64 ± 0.6 | 72 ± 0.3 | 1.00 | |
| Identified active chimeras (C) | C1 2 2 2 1 1 1 1 1 | 10 | 51 | 62 ± 0.5 | 69 ± 0.5 | 1.21 ± 0.07 |
| C2 2 2 1 1 2 1 1 1 | 7 | 52 | 50 ± 0.6 | 67 ± 0.4 | 0.94 ± 0.05 | |
| C3 1 1 1 1 1 2 2 2 | 9 | 52 | 64 ± 0.7 | 66 ± 1.0 | 0.92 ± 0.06 | |
| C4 2 2 1 1 1 2 1 1 | 14 | 55 | 64 ± 0.6 | 68 ± 0.9 | 0.84 ± 0.05 | |
| C5 2 1 1 1 1 1 1 1 | 8 | 21 | 64 ± 0.9 | 77 ± 0.8 | 1.42 ± 0.07 | |
| C6 1 1 1 1 1 1 2 1 | 9 | 24 | 60 ± 1.0 | 68 ± 0.6 | 1.34 ± 0.08 | |
| C7 2 2 1 1 1 1 1 1 | 6 | 41 | 58 ± 1.1 | 64 ± 1.2 | 1.35 ± 0.06 | |
| C8 2 2 2 2 1 1 1 1 | 8 | 63 | 68 ± 0.9 | 80 ± 1.4 | 1.23 ± 0.06 | |
| Predicted active and stable chimeras (C) | C9 1 1 2 2 2 2 1 1 | 22 | 51 | 67 ± 1.2 | 76 ± 1.1 | 1.18 ± 0.04 |
| C10 1 1 2 2 1 2 1 1 | 21 | 40 | 76 ± 1.1 | 83 ± 0.8 | 1.43 ± 0.09 | |
| C11 1 1 2 2 1 1 1 1 | 12 | 26 | 70 ± 0.6 | 81 ± 0.9 | 1.40 ± 0.07 | |
| C12 2 1 2 2 2 2 2 2 | 11 | 20 | 70 ± 0.4 | 74 ± 0.5 | 1.37 ± 0.06 | |
| C13 2 2 2 2 1 2 2 2 | 0 | 11 | 71 ± 1.0 | 73 ± 0.6 | 1.39 ± 0.04 |
1 TA50 is the temperature at which an enzyme has 50% of its optimal activity.
2 T50 is the temperature at which an enzyme loses 50% of its activity after 10 min pre-incubation.
3 Are is the cellulase’s specific activity at its optimal temperature measured in a 10 min assay with 1 μg purified enzyme and 1% PASC. The values are normalized relative to the specific activity of BsCel5A catalytic core.
* All of these enzymes are assayed at their optimal temperature at 60°C except that the optimal temperatures of C2 and C3 are 55°C; and P2, C5, C6, C7, and C12 are 50°C.