Regional, background subtracted NaFl permeability after 10, 40 and 90 days diet maintenance. (A) At 90 days, WD-DIO rats had significantly increased NaFl levels in all measured fields in dorsal hippocampus, compared to CHOW rats. (B) At 90 days, WD-DIO rats had significantly elevated NaFl levels in all ventral hippocampal cell fields, compared to CHOW, and elevated ventral CA2 NaFl compared to WD-DR rats. (C) At 90 days, there were no significant differences in total or ventral striatal NaFl expression, but WD-DIO rats showed significantly elevated dorsal striatal NaFl compared to CHOW. (D) There were no differences in NaFl permeability in the cerebellum at any time point. * indicates results significantly different from CHOW rats; ^ indicates results significantly different from WD-DR rats (* and ^ indicate p < 0.05; ** and ^^ indicate p < 0.01; *** and ^^^ indicate p < 0.001).