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. 2016 Jan 28;57(2):142–149. doi: 10.1093/jrr/rrv098

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© The Author 2016. Published by Oxford University Press on behalf of The Japan Radiation Research Society and Japanese Society for Radiation Oncology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Fig. 1. — ( A ) Representative total ion intensity in each fraction of mouse urine individually separated by high-performance liquid chromatography (HPLC): bold line, sham-irradiated; broken line, 24 h after 4 Gy irradiation. The sum intensity of each fraction is shown by retention time. Arrows show the changes in response to radiation. ( B , C and D ) Representative matrix-assisted laser desorption/ionization–time-of-flight mass spectrometry spectra of HPLC fractions no. 24, 35 and 46, respectively. The arrows represent the peak of angiotensin II ( m/z 1046) as the internal control, and the arrowheads represent m/z 1250 (B), 1721 (C) and 2821 (D), respectively. ( E ) Representative tandem mass spectrometry (MS/MS) spectra of m/z 1721 and (F) 2821. ( G ) Full MS/MS spectra of m/z 2821 were obtained from samples treated with dithiothreitol.