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( A ) Representative matrix-assisted laser desorption/ionization–time-of-flight mass spectrometry spectra from unfractionated urine, which we concentrated and deionized with OMIX tips. We collected urine samples from mice before irradiation (bottom) or 24 h (middle) or 48 h (top) after 4 Gy irradiation. Arrow: peptide fragment of uromodulin ( m/z 2001); arrowhead: hepcidin-2 ( m/z 2821). ( B ) Representative tandem spectrometry spectrum of m/z 2001.
