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. Author manuscript; available in PMC: 2017 Mar 16.
Published in final edited form as: Neuron. 2016 Mar 3;89(6):1223–1236. doi: 10.1016/j.neuron.2016.02.004

Figure 4. Mice derived from MT neurons.

Figure 4

(A) Newborn and (B) adult clones generated from MCNT-ES cells. (C) Standard and (D) fluorescence images of offspring of MCNT-mice. Transmission of the tdTomato transgene demonstrates MCNT-ES cells can generate functional germ cells. (E) Alternating standard and fluorescence images of brain, kidney, and heart dissected from Pcdh21/Cre-Ai9 control mice (top row) and MCNT-mice (bottom row). Organs from MCNT-mice exhibit normal morphology and uniform tdTomato expression. (F) Sample microsatellite PCR assay for tetraploid cell contribution to MCNT-mice. Band size distinguishes B2 derived cells from the tetraploid host strains C57 (C57BL/6J-Tyrc-2J) and Blb (Balb/cByJ). DNA titration curve demonstrates 5% detection limit. Analysis of DNA from B2 clone tissues exhibits no detectable tetraploid host DNA. M, molecular weight. E, B2 ES cell DNA. Br, brain. K, kidney. S, spleen. See also Figure S5.

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