Table 1.

Experimental protocols tested in the present study.

Protocols	Volume (nl)	NaCl (M)	Tip ∅ (μm)	Tip R. (MΩ)	Volt./Amp.	Frequency (Hz)	Pulse length	Total time	Cycles	N° Expts	Cases with labeling
Pr1	–	–	1–10	4–9	10–200 nA	0.5	1 s	5 s–10 m	1	9	0
Pr2	–	–	1–10	4–9	–50 V	1	50 ms	5 s	2–5	7	0
Pr3	–	–	1–10	4–9	–50 V	200	1 ms	1 s	2–5	7	0
Pr4	50–100	–	18–30	3–4	10–200 nA	0.5	1 s	5 s–10 m	1	10	0
Pr5	50–100	–	18–30	3–4	–50 V	1	50 ms	5 s	2–5	13	1
Pr6	50–100	–	18–30	3–4	–10 V	1	50 ms	5 s	2–5	5	1
Pr7	50–100	–	18–30	3–4	–50 V	200	1 ms	1 s	2–5	15	2
Pr8	50–100	–	18–30	3–4	–10 V	200	1 ms	1 s	2–5	5	0
Pr9	50–100	–	18–30	3–4	–80 V	200	1 ms	1s	2–5	5	0
Pr10	50–100	0.5	18–30	1–2	–	–	–	–	–	13	7
Pr11	50–100	0.5	18–30	1–2	–50 V	200	1 ms	1 s	2–5	28	24
Pr12	50–100	–	18–30	1–2	–	–	–	–	–	11	1

RNA delivery protocols tested in this study. “Total time” indicates the total period for which current (on/off cycles) were applied.