Table 2.
Summary of small RNA reads from two cassava brown streak viruses in three different cassava genotypesa and effect of the viruses on AGO2, DCL2 and DCL4 expression.
| Genotype | Virus species | Mean CBSD severity 6 WAG (1–5)b | Total raw reads | Total clean reads | Small RNAs mapped to virus genome | Percent vsRNAs with respect to total clean reads | Effect of virus on AGO2, DCL2 and DCL4 expression |
||
|---|---|---|---|---|---|---|---|---|---|
| AGO2 | DCL2 | DCL4 | |||||||
| TME204 | CBSV | 3.3 | 3,185,175 | 100,750 | 17,470 | 7.340 | up | down | up |
| UCBSV | 2.0 | 3,994,736 | 1,282,370 | 5,488 | 0.428 | up | down | down | |
| Healthy | 1.0 | 2,740,771 | 86,524 | 13 | 0.015 | na | na | na | |
| 60444 | CBSV | 4.3 | 3,198,984 | 130,400 | 16,496 | 12.650 | na | down | down |
| UCBSV | 3.0 | 4,020,006 | 612,987 | 15,939 | 2.600 | up | up | up | |
| Healthy | 1.0 | 3,347,340 | 10,238 | 12 | 0.117 | na | na | na | |
| NASE3 | CBSV | 5.0 | 3,019,728 | 188,617 | 18,705 | 9.917 | up | down | up |
| UCBSV | 1.0 | 2,511,404 | 410,641 | 10 | 0.002 | up | down | up | |
| Healthy | 1.0 | 2,154,804 | 213,163 | 10 | 0.005 | na | na | na | |
a
Data presented are an average of three biological replicates per genotype.
b
CBSD symptoms on grafted plants were scored as previously described (Ogwok et al., 2012). na = not applicable.