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. 2016 Mar 18;7:319. doi: 10.3389/fpls.2016.00319

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Copyright © 2016 Liu, Wang, Wang, Chen, Pang and Han.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Six primer pairs amplify the different sizes of sequences from Yangshen Baofei Pills. Except for the first lane (Marker), each primers pairs takes up four lanes. Lanes from left to right is 11F/12R, 11F/11R, 6F/4R, 7F/7R, and 4F/4R. The first lane in each primers pair group (lane 1, 5, 9, 13, and 17) is negative control. The fourth lane (lane 4, 8, 12, 16, and 20) is positive control. The other two lanes are PCR products from Yanshen Baofei Pills.