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Exposure to HH for 7 wk trends toward a significant induction of IL-18 mRNA in the RV (P = 0.08) compared with CO. IL-18 expression was assessed in all WT RV by real-time RT-PCR and normalized to 18S. Fold change was calculated by 2−ΔΔCt. IL-18 expression was undetectable in the RV from KO mice.
