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HH exposure for 7 wk does not significantly alter phosphorylation status of myofilament proteins, nor is phosphorylation status different between WT and IL-18 KO mice. Phosphorylation status was determined by Pro-Q Diamond staining of phosophoproteins relative to Coomassie brilliant blue staining of total protein. Open bars, CO; filled bars, HH. MLC1, myosin light chain 1; TnI, troponin I; MLC2, myosin light chain 2; TnT, troponin T.
