Fig. 7.

Chamber-specific upregulation of IL-2 and SDF-1 protein expression in IL-18 KO mice in response to 7 wk of HH. A: in the RV, IL-18 KO mice expressed higher IL-2 than WT mice. IL-2 was significantly induced upon HH in KO mice, whereas no effect was observed in WT (P value for interaction = 0.05). B: no significant differences in IL-2 expression were observed between genotypes or CO and HH within the left ventricle (LV). C: the interaction between genotype and environment trended toward significance in the RV, and calculation of the ratio between HH/CO indicated an induction of SDF-1 expression in KO mice but not in WT. D: SDF-1 expression trended to decrease in response to HH in both genotypes of the LV. IL-2 and SDF-1 expression was assessed by immunoblotting in the RV and LV and normalized to TnI. Open bars, CO; filled bars, HH. *P ≤ 0.05. All four conditions were run on the same gel, and nonessential lanes were cropped for generation of the representative images.