Fig. 3.

Comparison of inflammatory markers between the standard dosing model and repeated dosing model. Eight-week-old male FVB mice were injected (intraperitoneally) with saline vehicle or 25 mg/kg cisplatin given once (standard model) or with cisplatin (7 mg/kg) once a week for 4 wk (repeated dosing model). Animals were euthanized 72 h after the last injection. mRNA levels of TNF-α (A), IL-6 (B), IL-1β (C), monocyte chemotactic protein (MCP)-1 (D), and chemokine (C-X-C motif) ligand (CXCL)1 (E) were assessed in the kidney cortex via real-time quantitative RT-PCR and were normalized to their vehicle control. Data are expressed as means ± SE; n = 10. Statistical significance was determined by Student's t-test. *P < 0.05; **P < 0.01; ***P < 0.001.