Fig. 4.

Notch signaling is impaired in TβRII^−/− PT cells. A–E: qPCR was used to quantify transcription of Sp1, Sp3, Jagged1, Hes1, and Hey1. F: TβRII^flox/flox and TβRII^−/− PT cells were fractionated into cytosolic and nuclear components (see methods), and cleaved Notch1 (cNotch1) was measured with GAPDH and poly(ADP-ribose)polymerase 1 (PARP1) as loading controls for cytosolic and nuclear compartments, respectively. G: nuclear expression of cleaved Notch1 in TβRII^flox/flox and TβRII^−/− PT cells (n = 3). Values are means ± SE. *P < 0.05.