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. 2015 Dec 30;310(6):C456–C469. doi: 10.1152/ajpcell.00185.2015

Fig. 3.

Fig. 3.

91/130 competes with endogenous GBF1. HeLa cells were transfected with GFP-794 or GFP-91/130/794 and after 24 h processed by IF to detect GFP and the GM130 Golgi marker. A: GFP-794 targets to the Golgi when expressed at low levels (cell marked with arrow) but causes Golgi disruption when expressed at higher levels (cell marked with asterisk). B: GFP-91/130/794 also targets to the Golgi when expressed at low levels (cells marked with arrows). C: GFP-91/130/794 also causes Golgi fragmentation when expressed at higher levels (cells marked with asterisks). Both GFP-794 (A) and GFP-91/130/794 (C) colocalize with GM130 in the dispersed Golgi fragments. Bars, 10 μm.