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91/130 supports recruitment of Arf-dependent proteins. HeLa cells were transfected with BFA-resistant GFP-795 or GFP-91/130/795. After 24 h, cells were treated with 0.5 μg/ml BFA for 30 min and then processed by IF to detect the GFP tag and the indicated marker proteins. Cells expressing GFP-795 or GFP-91/130/795 recruit FAPP2 (A), MINT3 (B), Golgin-160 (C), and BIG1 (D) to membranes, while in untransfected cells the proteins are dispersed throughout the cell. Bars, 10 μm.
