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. 2015 Dec 30;310(6):C456–C469. doi: 10.1152/ajpcell.00185.2015

Fig. 7.

Fig. 7.

91/130 binds Arf1. A: proximity ligation assay between endogenous p115 and GM130 shows strong perinuclear Golgi signal. Insets indicate the Golgi localization of endogenous p115 and GM130 in the same experiment. B and C: HeLa cells were cotransfected with hemagglutinin (HA)-tagged Arf1 and either GFP-GBF1 (B) or GFP-91/130 (C). After 24 h, proximity ligation assays were performed with anti-HA and anti-GFP antibodies. Strong perinuclear Golgi signal is observed for both constructs and Arf1. Insets show the localization of HA-Arf1 and GFP-GBF1 or GFP-91/130 in the same experiment. Bars, 10 μm.