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. 2016 Mar 15;7:10997. doi: 10.1038/ncomms10997

Figure 5. Arp2/3-nucleated perinuclear actin facilitates nuclear squeezing through constrictions by weakening the lamin A/C network.

Figure 5

Representative images of immunostained DCs (a), (b) in 2-μm-wide constrictions and (c) in 8-μm-wide channels. White arrows indicate region of ruptured lamin A/C or Lamin B1 network. Scale bars10 μm (a,b (left panel) and b); 5 μm (a,b (right panel)). (d) Percentage of DCs showing a ruptured lamina network in 2-μm-wide and 15- or 5-μm-long constrictions, in straight 8-μm-wide channels and on two-dimensional substrates. (e) Histogram of the perimeter of the ruptured lamina region in 2-μm-wide and 15-μm-long constrictions. (f) Percentage of passage and (g) passage time in 2-μm-wide constrictions of DCs treated as indicated. (h) LifeAct-GFP (green)-expressing DCs stained with Hoechst (red). Left: control siRNA; right: laminA/C siRNA. Scale bar, 15 μm. (i) Percentage of passing cells with F-actin accumulation in 2-μm-wide constrictions. siCtrl, control siRNA; siLMNA, Lamin A/C siRNA. (d,f,g,i) Numbers represent the number of cells observed. Error bars are s.e.m. ***P-value<0.0001, **P-value<0.001 and *P-value<0.01. NS, nonsignificant. Statistical test: Fisher's test for c and f, Mann–Whitney test for d. L, constriction length; W, constriction width.. N≥3.