Fig. 3.
Effect of aspirin on the SSAT enzyme activity. Cells were seeded at a density of 2.4 × 104/cm2 on a 60 mm tissue culture dish in duplicate. After 48 h cell growth, the medium was replaced with fresh medium containing aspirin (2 mM). SSAT activity was assayed after 48 h drug exposure. a Effect of aspirin on SSAT activity in SSAT− cells. b Effect of aspirin on SSAT activity in SSAT+ cells. Values were mean ± SEM (n = 3 with 4 replicates per experiment). Statistical analysis was performed using two-way ANOVA with Bonferroni post-tests. *p < 0.05, **p < 0.01, ***p < 0.001