Fig. 4.
Effect of aspirin on polyamine content in SSAT− and SSAT+ cells. Cells were seeded at a density of 2.4 × 104/cm2 on a 60 mm cell culture dish in duplicate. Media were replaced after the initial 24 h growth and then at a 48 h interval. Cells were treated with aspirin (2 mM) after 48 h incubation and harvested at a 24 h interval; polyamine fraction was extracted by perchloric acid and finally quantified by LC–MS-MS. a N 1-acetylspermidine; b N 1-acetylspermine; c Putrescine; d Spermidine; e Spermine; f Total polyamines. Values are mean ± SEM with n = 3–6, two duplicates per experiment). Statistical analysis was performed using Two-way ANOVA with Bonferroni post-tests comparing with Control. *p < 0.05; **p < 0.01; ***p < 0.001