Table 2.
Time in culture (h) | 24 | 48 | 72 | 96 |
---|---|---|---|---|
(A) | ||||
SSAT− | 1.56 ± 0.38 | 1.58 ± 0.23 | 1.30 ± 0.29 | 1.27 ± 0.20 |
SSAT+ | 3.89 ± 0.47*** | 5.73 ± 0.65*** | 3.35 ± 0.20*** | 3.48 ± 0.38*** |
Time of treatment (h) | SSAT− | SSAT+ | ||
---|---|---|---|---|
Control | Aspirin (2 mM) | Control | Aspirin (2 mM) | |
(B) | ||||
24 | 0.96 ± 0.13 | 0.18 ± 0.08*** | 2.74 ± 0.28 | 1.70 ± 0.25** |
48 | 0.63 ± 0.14 | 0.39 ± 0.14 | 1.16 ± 0.15 | 0.49 ± 0.10 |
Cells were seeded at a density of 2.4 × 104/cm2 in a 60 mm cell culture dish in duplicate. (A) The cells were harvested at a 24 h interval till 96 h in culture. (B) After 48 h incubation, the cells were treated with 2 mM aspirin for 24 and 48 h. Results shown are mean ± SEM (n = 3, with 2 replicate per experiment). Statistical analysis was performed by 2-way ANOVA with Bonferroni post-tests using Prism 5. ** p < 0.01, *** p < 0.001