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. 2015 Dec 24;48:1003–1012. doi: 10.1007/s00726-015-2143-6

Table 2.

ODC enzyme activity in SSAT and SSAT+ cells

Time in culture (h) 24 48 72 96
(A)
 SSAT 1.56 ± 0.38 1.58 ± 0.23 1.30 ± 0.29 1.27 ± 0.20
 SSAT+ 3.89 ± 0.47*** 5.73 ± 0.65*** 3.35 ± 0.20*** 3.48 ± 0.38***
Time of treatment (h) SSAT SSAT+
Control Aspirin (2 mM) Control Aspirin (2 mM)
(B)
 24 0.96 ± 0.13 0.18 ± 0.08*** 2.74 ± 0.28 1.70 ± 0.25**
 48 0.63 ± 0.14 0.39 ± 0.14 1.16 ± 0.15 0.49 ± 0.10

Cells were seeded at a density of 2.4 × 104/cm2 in a 60 mm cell culture dish in duplicate. (A) The cells were harvested at a 24 h interval till 96 h in culture. (B) After 48 h incubation, the cells were treated with 2 mM aspirin for 24 and 48 h. Results shown are mean ± SEM (n = 3, with 2 replicate per experiment). Statistical analysis was performed by 2-way ANOVA with Bonferroni post-tests using Prism 5. ** p < 0.01, *** p < 0.001