Fig. 8.

Effect of IL-1β on ROS generation. Dihydroethidium (DHE) staining was used to detect ROS generation in the aorta. A: aortas from NT mice were pretreated with or without apocynin (0.3 mM) for 1 h before IL-1β (500 pg/ml, 24 h) or control treatments (n = 6). B: aortas from NT or E-WT were treated with IL-1β (500 pg/ml, 24 h) alone (n = 7). Samples labeled 1 and 2 refer to two independent replicates showing the range of DHE staining in the experiments. All data are means ± SE. *P < 0.05, IL-1β vs. no treatment or apocynin + IL-1β.