Table 3.
Antimicrobial activity and MIC (μg/mL) of Nocardia sp. PB-52 by broth dilution method.
| Test microorganisms | *Zone of inhibition (mm) | MIC of EA-PB-52 (μg/mL) | MIC of Rif (μg/mL) | MIC of Strep (μg/mL) | MIC of Amp B (μg/mL) |
|---|---|---|---|---|---|
| GRAM-POSITIVE BACTERIA | |||||
| S. aureus MTCC 96 | 36k±0.8 | >0.975 | >1.95 | >6.25 | NA |
| S. aureus MTCC 3160 | 29hij±0.8 | >1.95 | >1.95 | >12.5 | NA |
| S. epidermidis MTCC 435 | 23de±1.6 | >7.81 | >3.125 | >6.25 | NA |
| B. subtilis MTCC 441 | 28ghi±0.8 | >1.95 | >1.95 | >6.25 | NA |
| B. cereus MTCC 1272 | 22d±0.4 | >7.81 | >6.25 | >12.5 | NA |
| B. megaterium MTCC 8075 | 26fg±0.4 | >3.9 | >3.12 | >3.12 | NA |
| M. luteus MTCC 1538 | 22d±1.6 | >7.81 | >0.97 | >6.25 | NA |
| MRSA ATCC 43300 | 30ij±0.8 | >1.95 | >25 | >50 | NA |
| GRAM-NEGATIVE BACTERIA | |||||
| E. coli MTCC 40 | 31j±1.2 | >3.9 | >6.25 | >3.12 | NA |
| E. coli MTCC 739 | 25ef±0.4 | >3.9 | >50 | − | NA |
| S. marcescens MTCC 97 | 18b±1.2 | >31.2 | >12.5 | >3.12 | NA |
| K. pneumoniae MTCC 3384 | 27fgh±1.6 | >1.95 | >25 | − | NA |
| K. pneumoniae ATCC 13883 | 15a±1.6 | >62.5 | >50 | − | NA |
| P. aeruginosa MTCC 741 | 29hij±0.8 | >1.95 | >25 | >25 | NA |
| P. aeruginosa MTCC 424 | 19bc±1.2 | >15.6 | − | >12.5 | NA |
| P. aeruginosa MTCC 2582 | 26fg±0.8 | >3.9 | >50 | >25 | NA |
| P. vulgaris MTCC 426 | 17a±0.8 | >31.25 | >25 | >6.25 | NA |
| YEAST | |||||
| C. albicans MTCC 227 | 27fgh±0.4 | >1.95 | NA | NA | >0.97 |
| C. tropicalis MTCC 2208 | 23de±0.2 | >7.81 | NA | NA | >0.48 |
| C. albicans ATCC 10231 | 21cd±1.6 | >7.81 | NA | NA | >1.95 |
*
Zone of inhibition by spot inoculation method on GLM agar medium. Average size of colony of PB-52 in GLM agar was (7 ± 2) mm in diameter after 8 days of incubation at 28°C.
Zone of inhibition values are given as mean ± SD (n = 3). Values having different superscripts (a-k) differ significantly (P < 0.05).
Rif, Rifampicin (antibacterial agent); Strep, Streptomycin (antibacterial agent); Amp B, Amphotericin B (antifungal agent); NA, not applicable; −, No activity.