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. 2015 Nov 17;94(3):285–292. doi: 10.1038/icb.2015.91

Figure 2.

Figure 2

The CnB–TLR4 interaction results in cytokine production through IκB-α phosphorylation. (a), Quantitative PCR for NF-κB (2.1-fold) and IκB-α (9.3-fold). P<0.001, N=3. (b) Western blot to detect IκB-α (with or without phosphorylation) and IL-8 in CnB-treated (lane 2) or buffer solution-treated (lane 1) U937 cells; the cells were stimulated with CnB or its buffer solution for 3 h. (c) Western blot to detect IκB-α phosphorylation at various time points in CnB-treated U937 cells. (d) Western blot to detect IκB-α phosphorylation in U937 cells treated with different antibodies. (e and f) ELISA for IL-8 and TNF-α in HEK293 cells transfected with TLR4 and/or MD2 and treated with CnB and LPS for 3 h. The error bars represent the mean±s.d.