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. 2015 Dec 4;310(3):H376–H384. doi: 10.1152/ajpheart.00748.2015

Fig. 2.

Fig. 2.

MDL prevents cytosolic calpain activation during IR. A: spectrin is a substrate of calpain, and an increase of its cleavage product is commonly used as an marker of calpain activation in cytosol. IR increased the cleaved spectrin content (150 kDa) in cytosol compared with TC. In contrast, MDL treatment decreased spectrin cleavage during IR compared with untreated hearts. Values are means ± SE; n = 3 in each group. *P < 0.05 vs. TC or MDL-treated mitochondria. B: in isolated control mouse heart mitochondria, MDL decreased mitochondrial calpain 1 (mit-CPN1) activity in a dose-dependent manner. The fluorescence intensity in mitochondria without MDL is 5,471 ± 185 arbitrary units (n = 6), and this value was used as 100%. Values are means ± SE; n = 3 and 5 in MDL 3 μM and 10 μM groups, respectively. *P < 0.05 vs. mitochondria without MDL treatment. †P < 0.05 vs. 3 μM MDL group.