Fig. 4.

Microglial inhibition decreases ANG II-increased ROS production within the PVN of rats. A–C: confocal images showing hypothalamic slices incubated in DHE in the presence of aCSF (A); ANG II (1 μM; B) and ANG II + minocycline (Mino; 100 μM; C). D: summary data showing increased ROS production in the presence of ANG II and the blunted effect observed in slices pretreated with minocycline. Confocal images represent data obtained 8 min after the beginning of the acquisition. Scale bars = 50 μm. n = 4 rats/group. ***P < 0.0001 vs. aCSF and +++P < 0.0001 vs. ANG II at each given time point. (Main effect by two-way ANOVA: treatment: F = 17.3, P < 0.0001; time: F = 4.5, P < 0.0001; interactions: F = 2.93, P < 0.0001.)