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. 2016 Mar 9;6:22452. doi: 10.1038/srep22452

Figure 2. EGFR expression of OSCC cells and affinity of EGFR-binding peptide for OSCC cells.

Figure 2

(A) EGFR expression levels in whole -cell lysates of HEK293 and OSCC cells (TE-5, TE-8, TE-10, TE-11, TE-5R, and TE-11R). The EGFR protein expression level was determined by Western blotting. β-Actin served as a loading control. EGFR protein was highly expressed in all OSCC cells, while its level was very low in HEK293 cells. (B) EGFR expressions on the cell surface of HEK293 and OSCC cells. Cells were stained with AlexaFluor 647-conjugated monoclonal anti-EGFR antibody or IgG isotype control antibody. The Fluorescence intensity determined by flow cytometry showed that EGFR was expressed on the surface of OSCC cells, whereas HEK293 cells minimally expressed it. (C,D) Affinity of EGFR-binding peptide for HEK293 and TE-11R cells. They were exposed to Fluorescein (FLC)-labelled EGFR-binding peptide, and the fluorescence intensity was assessed by flow cytometry. The Affinity of EGFR-binding peptide for cell membranes of TE-11R cells is increasing in time- (C) and dose- (D) dependent manners, but not in HEK293 cells. The assays were repeated three times, and data are shown as the mean ± SD. n.s.: not significant, *P < 0.05, **P < 0.01 vs. untreated (0 minutes) cells.