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. 2016 Mar 18;6:23241. doi: 10.1038/srep23241

Figure 1. Identification of binding partners for the GPNMB extracellular fragment.

Figure 1

To identify novel interacting proteins of GPNMB, we conducted a comprehensive search using Membrane Protein Library (MPL)/BLOTCHIP-MS technology. MPL enables users to extract all cellular membrane proteins and reconstitute them in artificial lipid bilayers composed of phospholipid liposomes to protein function. By differential analysis of SDS-PAGE electrophoresis profile the MPLs, three bands were detected at about 35, 60, and 120 kDa and were considered possible receptors.