Figure 2.

Prox1 heterozygosis affects the formation of ADMs in the context of oncogenic Kras activity.

A: Day 0, Acinar cells (arrowheads) from KC pancreata express amylase, but no CK19 or Prox1, at day 0 of culture (arrows indicate CK19⁺/Prox1⁺ centroacinar cells). Day 3, KC acini cultured for 3 days with TGFα form cysts (arrows) that are negative for amylase and positive for CK19 and Prox1.

B, C: Prox1 heterozygosis increases the size of Kras^G12D-expressing acinar-derived cysts after 3 days of culture with TGFα.

E: Quantitative PCR results show higher expression of Cldn18 transcripts in KCH versus KC acinar cultures treated for 3 days with TGFα. Error bars represent + SEM values (n=3-4 individual specimens per time point).

E, F: Prox1 heterozygosis increases the number and proliferation rate of Kras^G12D-expressing acinar-derived cysts cultured for 3 days with TGFα.

*P< 0.05, **P< 0.01 and *** P< 0.001.