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. 2016 Mar 18;6:23426. doi: 10.1038/srep23426

Figure 4. Enhanced β-adrenergic signalling in WAT of Ahnak−/− mice.

Figure 4

(A) CL-316243 stimulated glycerol levels in serum from RC-fed mice (20 min after CL injection) (n = 5). (B) Fold changes of glycerol levels over basal level after CL316243 stimulation in serum (n = 5). (C) Serum epinephrine levels (n = 7–9 for basal, n = 17–20 for CL). (D) Relative mRNA levels of Adrb3, assessed by qRT-PCR in CL-treated adipose tissue (n = 5); the expression was normalized to that of 36B4. (E,F) Levels of phosphorylated HSL and PKA substrates assessed by immunoblotting in eWAT (E) and iWAT (F) GAPDH was used as a loading control. (G,H) CL-316243 stimulated glycerol (G) and FFA (H) levels in serum from HFD-fed mice (20 min after CL injection) (n = 4–5). The data are presented as the mean ± SEM; *P < 0.05, **P < 0.01, ***P < 0.001, wild-type (WT) versus Ahnak−/− (KO) mice.