Figure 5. ACSLs are recruited into the C. trachomatis (Ct) inclusion in the absence of lipid droplets (LD).

Control and T863 treated DGAT2−/− MEF cells were infected with Ct L₂ without removing the inhibitor from the media. After 24 h, cells were fixed and labeled with anti-Ct MOMP antibody (green), anti-ACSL3 (A) or anti-ACSL4 (B) antibodies (red), and Hoechst to stain nuclear and bacterial DNA (blue). Representative images of z-stack projections from confocal microscopy are shown. White lines indicate localization of ACSLs within the Ct inclusion in the three planes, x, y, and z. Scale bar, 5 μm.