Figure 7. The transport of ACSLs into the C. trachomatis (Ct) inclusion is independent of the exocytic pathway.

(A) HeLa cells were infected with Ct L₂ and were either treated with Brefeldin A (BFA) from 2–4 hpi (right) or left untreated (left). After 24 hpi, cells were fixed and prepared for confocal microscopy. The inclusion was labeled with anti-Ct MOMP antibody (green), anti-human golgin 84 antibody (red), and Hoechst for nuclear and bacterial DNA (blue). Scale bar, 10 μm; (B) HeLa cells were infected with Ct L₂ and treated with Brefeldin A (BFA) from 2–4 hpi. After 24 hpi, cells were fixed and prepared for confocal microscopy. The inclusion was labeled with anti- Ct MOMP antibody (green), anti-human ACSL-specific antibodies (red), and Hoechst for nuclear and bacterial DNA (blue). Representative images of z-stack projections from confocal microscopy are shown. White lines indicate localization of the ACSLs inside the inclusion in the three planes, x, y, and z. Scale bar, 5 μm.