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. 2016 Mar 18;6:23278. doi: 10.1038/srep23278

Figure 3. Treg cell induction by HUVECs was mediated through secretion of soluble factors.

Figure 3

CD4+CD25−Foxp3− T cells were co-cultured with primary female or male HUVECs at a cell-to-cell ratio of 1:1 (T cells:HUVECs) in a trans-well system. After 48 and 72 hours, the number of CD4+Foxp3+ T cells was analyzed. Female (a) and male (b) HUVECs stimulated Treg cell generation to a different extent. Co-cultures with HUVECs are presented as fold change of controls (T cells cultured alone). Female HUVECs showed a significant higher capacity to induce Treg cells (c). Samples from three normal pregnant women were analyzed in duplicates. Data are presented as means ± S.E.M. Statistical analysis among groups was performed using Two-way-ANOVA followed by Bonferroni correction for multiple comparisons. *p < 0.05.