Table 6. Optimized conditions suggested by Design Expert.
| Solution | A | B | C | D | Yield (g FW/L) | PSL content (%) | COGS (€/kg) |
|---|---|---|---|---|---|---|---|
| S0 | 26 | 7 | 20 | on | 146 | n.d. | 125 |
| S3 | 23 | 13 | 10 | on | 407 ± 10/366* | 5 ± 1/5* | 45 |
| S4 | 23.5 | 9 | 10 | on | 392 ± 30/336* | 5 ± 0/12.5* | 47 |
Several different solutions were suggested by Design Expert, and the two best examples (S3, S4) are shown here compared to the starting conditions (S0). Values for yield and PSL content marked in italic and with an asterisk represent the predicted values from Design Expert. The first value represents the measured yield/content. See Supplement 2 for details regarding the COGS calculation. n = 6 biological replicates
A: temperature; B: incubation time; C: inoculum density; D: light.