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. 2015 Nov 10;10(4):810–822. doi: 10.1038/ismej.2015.155

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Copyright © 2016 International Society for Microbial Ecology

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(a) Hydrogen production assay demonstrating low background levels of anerobic hydrogen production without any carbon addition. Acetate addition produces elevated hydrogen production. Hydrogen production after acetate addition may be owing to the activity of a cytoplasmic hydrogen dehydrogenase restoring the NADH/NAD imbalance caused by glycogen degradation anerobically. (b) Batch tests were conducted to test the viability of glycine as a carbon source for Accumulibacter. Phosphorus release after carbon contact was measured for acetate, glycine and a no carbon addition control. These results demonstrate that glycine addition stimulates phosphorus release and is therefore a viable carbon source for Accumulibacter.